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Time-resolved spectral multiplexing for proteomic applications

Reference	BB/C51287X/1
Principal Investigator / Supervisor	Professor C Morgan
Co-Investigators / Co-Supervisors	Dr Andrew Mitchell, Professor Huw Rees
Institution	University of Salford
Department	Environment and Life Sciences
Funding type	Research
Value (£)	392,320
Status	Completed
Type	Research Grant
Start date	01/02/2005
End date	30/04/2008
Duration	39 months

Abstract

Recent advances in time-resolved fluorescence detection have resulted in CCD imaging systems that have nanosecond timing capability when used with pulsed laser sources. The new detectors are physically small and robust yet have very high sensitivity and excellent dynamic range combined with very low noise levels. Some CCD detectors have integral colour filter masks allowing them to be used for true-colour imaging and it is proposed to combine this capability with fast time-resolution to produce an imaging system suited to measurement of fluorescently stained proteins in electrophoresis gels used in proteomic applications. Two new imaging systems will be tested. A fixed-format imager is well-suited to experiments with small gels while larger gels are best measured using a hybrid scanned imager which optimises spatial resolution and sensitivity. The technology will be used to investigate a panel of labels chosen on the basis of spectral properties and decay time of emission as candidates for multiplexed detection. The ability to detect a range of labels simultaneously on a stained electrophoresis gel and on Western Blots offers substantial potential advantages over current practice and joint spectral/temporal multiplexing would greatly increase the throughput of proteomics measurements.

Summary

unavailable

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	Technology and Methods Development
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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