BBSRC Portfolio Analyser
Award details
The relationship between patterns of gluten protein synthesis and deposition and grain processing quality: a transgenic approach
Reference
BB/C512710/1
Principal Investigator / Supervisor
Professor Peter Shewry
Co-Investigators /
Co-Supervisors
Dr Alison Huttly
,
Professor Huw Jones
,
Professor Johnathan Napier
,
Dr Paola Tosi
Institution
Rothamsted Research
Department
Plant Biology & Crop Science
Funding type
Research
Value (£)
297,238
Status
Completed
Type
Research Grant
Start date
14/11/2005
End date
13/05/2010
Duration
54 months
Abstract
Transgenic wheat will be used as a tool to study the relationship between the spatial patterns of gluten protein gene expression in the grain, the pathways of gluten protein trafficking and deposition and grain processing. 1. Bread wheat cv Cadenza will be transformed to express epitope-tagged forms of three major types of gluten protein (-gliadin, LMW subunits, HMW subunits) under the control of promoters which confer expression in the peripheral and central parts of the starchy endosperm. 2. the pathways of trafficking and deposition of the three proteins will be determined in the two regions of the developing starchy endosperm, using immunogold labelling. 3. three-dimensional distributions of total proteins and the epitope tagged proteins in the developing grain will be determined by electron tomography of serial sections. 4. the effects of the transgenes on the formation of glutenin polymers and dough mixing strength will be determined by analysis of flour fractions derived from the peripheral cells of the starchy endosperm, the central cells of the starchy endosperm and the whole starchy endosperm.
Summary
unavailable
Committee
Closed Committee - Agri-food (AF)
Research Topics
Crop Science, Plant Science
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
I accept the
terms and conditions of use
(opens in new window)
export PDF file
back to list
new search