Award details

Differential display proteomics for Aberyswyth - multiplexing quantitation and statisical analysis.

Reference	BB/C511272/1
Principal Investigator / Supervisor	Professor Joanne Hamilton
Co-Investigators / Co-Supervisors	Professor John Barrett, Dr James Bell, Professor Glyn Jenkins, Dr Michael Rose, Dr Michael Winson
Institution	Aberystwyth University
Department	Inst of Biological, Environ & Rural Sci
Funding type	Research
Value (£)	25,000
Status	Completed
Type	Research Grant
Start date	01/01/2005
End date	30/09/2005
Duration	9 months

Abstract

This proposal requests new infrastructure support to purchase a multi-site licence for Nonlinear Dynamics Phoretix 2D Evolution software with Cross Stain Facility and PC controls for the expansion of functional genomic research in the multi-institution based Aberystwyth BioCentre (ABC). Research scientists within ABC are pioneering new developments in proteome technology through productive collaborations with industry (Nonlinear Dynamics and Pfizer) and incorporating proteomics to address Research Council funded problems in biosciences. The system requested is compatible with all current and emerging gel-based technologies and interfaces with current BBSRC/HEFCW/SRIF funded equipment including a 2 dye difference gel electrophoresis (2D-DiGE) proteomic system, a multiwavelength fluoroimager, spot cutter, and Micromass MALDI-ToF and ESI- MS/MS mass spectrometers. The most significant drawback of conventional two-dimensional gel electrophoresis proteomic analysis has been the poor reproducibility and the difficulties of quantitatively comparing multiple gels. These problems have largely been overcome with the introduction of 2D-DiGE, using both a standard 2D DiGE approach and more recently with the introduction of an internal standard for 2D-DiGE experiments. However a significant bottleneck in proteomic analysis has resulted from the lack of suitable software for the rapid and accurate analysis, quantitation and statistical comparison of gel data. The project between ABC and Nonlinear involves the development of the cross-stain platform for multiplexed gels. Collaborative efforts contributed to the initial development of the cross-stain analysis facility and the sustained collaboration will continue the development of this software. Our background in proteomics with a specialism in 2D-DiGE technology has allowed us to negotiate a significant collaboration with Nonlinear dynamics resulting in a 50 per cent contribution toward the purchase of the requested items. This advanced 2D gel analysis platform includes features to enable the analysis of multiplexed 2D gels, making it suitable for working with the full range of gel-based techniques. The package is compatible with CY Dyes trademark for 2D-DiGE, SYPRO trade mark dyes, the Pro-Q range for post translational modifications and traditional Coomassie and silver stains. The Phoretix Evolution package with this cross stain facility is unique in its ability to analyse and manage all of these techniques within a single 2D image analysis platform. The only other DiGE compatible software on the market cannot be used for the analysis of non-DiGE gels. The purchase of the items requested would provide a comprehensive and DiGE-compatible gel analysis platform for Aberystwyth researchers which will allow maximum proteomic data recovery through multiplexing, quantitation and statistical analyses. The proposal supports interdisciplinary research and collaboration and is driven by specific research council and other proteome projects in parasitology, microbiology, lactation sciences, reproductive physiology, plant physiology and marine and freshwater biology. The proteomics suite at Aberystwyth underpins a number of BBSRC funded functional genomic projects and other new pump priming research initiatives.

Summary

unavailable

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Research Equipment Initiative 2004 (RE4) [2004]
Funding Scheme	X – not Funded via a specific Funding Scheme

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