Award details

High throughput screening of sequence variation in crop and model plants

Reference	BB/C511056/1
Principal Investigator / Supervisor	Professor Kim Hammond-Kosack
Co-Investigators / Co-Supervisors	Professor Peter Hedden, Dr Konstantin Kanyuka, Professor Angela Karp, Dr Andrew Phillips
Institution	Rothamsted Research
Department	Plant Pathology and Microbiology
Funding type	Research
Value (£)	130,092
Status	Completed
Type	Research Grant
Start date	01/02/2005
End date	31/01/2006
Duration	12 months

Abstract

The equipment requested in this application will support programmes in several areas of genomic science across three Divisions of the Institute. In the Plant-Pathogen Interactions Division, Kim Hammond-Kosack and Kostya Kanyuka propose to identify novel variation in genes conferring resistance to plant pathogens in wheat and Arabidopsis. The diploid wheat species, Triticum monococcum, is being developed as a model for pathogen resistance and as a source for novel resistance. An EMS-mutagenised population is being developed that will be screened by TILLING for sequence variants in genes for known resistance signalling components. In addition, a collection of 123 T. monococcum accessions will be screened for sequence variation in these key genes and F2 mapping populations will be used to map the R loci between different resistant and susceptible accessions. In Arabidopsis, this group propose to use forward genetic screens to identify loci conferring altered resistance to Fusarium infection. These loci will be mapped and defined chromosome intervals will be screened by PCR TILLING to identify the mutations. In the Crop Performance Improvement Division, Andy Phillips and Peter Hedden also propose to exploit the T. monococcum EMS-mutagenised and natural populations to investigate the role of GA metabolism and signalling in key developmental processes in wheat, including performance characters such as stem height and grain expansion. The roles of individual paralogues of GA biosynthetic genes in controlling different aspects of wheat development will be investigated. It is also proposed to extend PCR TILLING to hexaploid bread wheat, targeting highly-expressed homoeologues of individual genes to manipulate GA signalling associated with specific processes. In the Plant and Invertebrate Ecology Division, Angela Karp is developing molecular markers for willow breeding, in an endeavour to improve willow varieties for renewable energy. A QTL linkage map for agronomically important traits has been produced and is being enriched to a high level with a variety of molecular markers. In addition, the genetic diversity of 1200 willow accessions will be assessed using molecular marker approaches. This approach is currently limited by the manual set up of routine assays and would be much assisted by laboratory automation. We therefore request assistance with the purchase of a capillary-based DNA sequencer and genotyper and a liquid handling robot for execution of repetitive tasks. In part, this will restore access to genomics equipment, which was lost in Professor Keith Edwards move to Bristol University on the closure of Long Ashton Research Station.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Research Equipment Initiative 2004 (RE4) [2004]
Funding Scheme	X – not Funded via a specific Funding Scheme

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