Award details

Quantitative dynamic intracellular imaging

Reference	BB/C511005/1
Principal Investigator / Supervisor	Dr Clive Price
Co-Investigators / Co-Supervisors	Professor Alistair Hetherington, Dr Martin Mcainsh, Dr Paul McKean, Mr Matthew Roberts, Dr Alan Shirras
Institution	Lancaster University
Department	Biological Sciences
Funding type	Research
Value (£)	57,193
Status	Completed
Type	Research Grant
Start date	22/11/2004
End date	21/11/2005
Duration	12 months

Abstract

The QLM will greatly enhance the capabilities of the recently commissioned Delta Vision Spectrisimage restoration system and extend the tools available to support a wide range of BBSRC funded research within the department. In the immediate the equipment would be deployed to support projects in yeast cell biology (Price), plant cell signalling (Hetherington, McAnish and Roberts), parasitology (McKean) and insect neuro- and developmental biology (Shirras). In all of these projects there is a need to move to real time quantitative cell biology techniques to fully examine the behaviour of the systems under study. The range of techniques to be enabled by the funding request include: fluorescence recovery after photobleaching (FRAP) and fluorescence loss in photobleaching (FLIP) two techniques that permit the examination of protein turnover and localisation and allow conclusions to be drawn about the movement of proteins within living cells e.g. whether observed protein movement can be explained by a diffusional mechanism or requires an active transport mechanism to be invoked; fluorescence energy resonance transfer (FRET) which allows the analysis of weak and transitory protein/protein interactions within subcellular structures and the distinction between co-localisation and direct protein/protein interaction within that structure as fluorescent energy resonance transfer is distance limited to the molecular level. A major advantage to having the QLM capability will be the ability to rapidly exploit new methods as they emerge. For example a method termed fluorescence localisation after photobleaching (FLAP) was developed last year to quantify rapid changes in the actin cytoskeleton during mammalian cell movement and this would be used immediately by the plant cell signalling group, as described below. For all of these projects addition of the QLM to the Delta Visin Spectris will allow these techniques to be applied under experimental conditions in which low emission light intensity detection is a prime consideration.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Research Equipment Initiative 2004 (RE4) [2004]
Funding Scheme	X – not Funded via a specific Funding Scheme

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