Award details

Utilisation of new technologies for sequencing genotyping and mutilation detection

Reference	BB/C510959/1
Principal Investigator / Supervisor	Professor Ian Bancroft
Co-Investigators / Co-Supervisors	Professor Michael Bevan, Dr Jonathan Clarke
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	193,602
Status	Completed
Type	Research Grant
Start date	01/01/2005
End date	30/06/2005
Duration	6 months

Abstract

Much of modern biological research is underpinned by molecular genetic approaches. As genome sequence data become available for wider ranges of species, including crops and useful microbes, the opportunities for advancing scientific understanding grow, as does the requirement for state-of-the-art analytical equipment. The identification of genes involved in the control of biological processes requires not only the ability to efficiently generate high quality DNA sequence data, but also the ability to exploit molecular marker systems for mapping and for the analysis of natural variation. The availability of complete genome sequences facilitates the use of reverse genetic approaches to identify mutations or allelic variants in candidate genes. These three key areas of sequencing, genotyping and mutation detection can be run on the same analysis platforms, which must give the highest quality results at the lowest achievable cost. The state-of-the-art for such a platform has moved on from the discontinued ABI 3700 to the ABI 3730. In addition, the technology for liquid handling has advanced recently, with a commercial system becoming available for the setting-up and processing of reactions of total volume below 1 microlitre. This system is the Parallab 350, which permits substantial cost saving by reducing reagent wastage, and can circumvent the problems associated with multiplex PCR by allowing the set-up of individual small-scale reactions that can subsequently be pooled for analysis. We propose to purchase an ABI 3730 and Parallab 350. However, purchase of equipment is only the first step in the reduction to practice of new technology. The JIC Genome Laboratory (JGL) is at the heart of the strategy of Norwich Research Park (NRP) for the exploitation of genomics. The mission of the JGL is to provide access to state-of-the-art genomic technologies and to provide hotel accommodation to scientists with large genomics projects. The 13 laboratory-based staff of JGL will establish the detailed protocols and develop the know-how to efficiently utilise the equipment, which will be used for the advancement of plant and microbial sciences at JIC. The benefit of the new technology will be felt across NRP and, as the JGL provide cost-recovered collaborative support to many additional institutions, across the UK.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Research Equipment Initiative 2004 (RE4) [2004]
Funding Scheme	X – not Funded via a specific Funding Scheme

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