Award details

Genomics of enterotoxigenic Escherichia coli (ETEC)

Reference	BB/C510508/1
Principal Investigator / Supervisor	Professor Julian Parkhill
Co-Investigators / Co-Supervisors
Institution	Wellcome Trust Sanger Institute
Department	Wellcome Trust Sanger Institute Department
Funding type	Research
Value (£)	218,663
Status	Completed
Type	Research Grant
Start date	13/05/2005
End date	12/05/2007
Duration	24 months

Abstract

The last half-century has seen a remarkable shift in our view of intestinal Escherichia coli, from one of harmless commensal to that of a versatile pathogen able to cause disease in both man and animals. E. coli may be catalogued into several pathogenic groups termed pathotypes. Sequencing projects have resulted in the complete genome sequences being available for all but one recognised pathotype of E. Coli enterotoxigenic E. coli (ETEC). Surprisingly, this pathotype is responsible for the majority of E. coli mediated intestinal disease, as well as causing significant economic losses as a pathogen of animals. Here we propose to sequence the complete genome of enterotoxigenic E. coli H10407 using a whole genome shotgun method. Once the sequence data is generated it will be annotated, made available through the BBSRC funded coliBase, and used to prime hypothesis-driven research. Furthermore, using multilocus sequence typing we will determine the phylogenetic distribution of ETEC. Strains of ETEC representative of each phylogenetic cluster will be examined by whole genome PCR and microarray to determine gene content and genetic structure, and more fully characterise the ETEC vaccine strains currently being used in clinical trials. (Joint with BB/C510075/1).

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

BB/C510075/1 Genomics of enterotoxigenic Escherichia coli (ETEC)

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