Award details

Regulation of cellular stress responses in the fungal pathogen Candida albicans - a proteomic approach

Reference	BB/C510391/1
Principal Investigator / Supervisor	Professor Al Brown
Co-Investigators / Co-Supervisors
Institution	University of Aberdeen
Department	School of Medical Sciences
Funding type	Research
Value (£)	179,257
Status	Completed
Type	Research Grant
Start date	01/10/2004
End date	30/09/2007
Duration	36 months

Abstract

Candida albicans is the major systemic fungal pathogen of humans. Its success as a pathogen is based partially upon its resistance to oxidative stresses and other environmental insults. However, the molecular mechanisms that underpin this resistance are poorly understood. Recent studies from our own (and other) laboratories have indicated that C. albicans have evolved specialised stress responses compared with budding and fission yeasts, and that Hog1 stress activated protein kinase (SAPK) plays a pivotal role in these responses. Our working hypothesis is that the Hog1 SAPK coordinates a specialised General Stress Response in C. albicans. We will test this hypothesis firstly by defining the effects of specific stresses upon the C. albicans proteome, and the role of Hog1 in mediating these responses. Specific proteins highlighted using proteomics will be examined. First, bioinformatics will be used to map their predicted functions onto metabolic and signalling pathways (where possible). Then their functions will be tested directly in C. albicans using reverse genetics to examine the stress and growth phenotypes of null mutant. In addition, proteomics will be combined with molecular and cellular approaches to identify and characterise upstream and downstream targets of Hog1. In this way we will generate a comprehensive picture of Hog1 mediated stress responses in this fungal pathogen. (Joint with BB/C510383/1)

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Proteomics and Cell Function (PCF) [2003-2004]
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

BB/C510383/1 Regulation of cellular stress responses in the fungal pathogen Candida albicans - a proteomic approach

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