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Elucidating the receptor like protein-mediated defence response in Arabidopsis using the downy mildew resistance gene RPP27

Reference	BB/C509490/1
Principal Investigator / Supervisor	Professor Mahmut Tör
Co-Investigators / Co-Supervisors	Professor Eric Holub
Institution	University of Warwick
Department	Warwick HRI
Funding type	Research
Value (£)	298,967
Status	Completed
Type	Research Grant
Start date	01/03/2005
End date	30/09/2008
Duration	43 months

Abstract

The mode of action of RLPs including the meristem development gene CLAVATA2 in Arabidopsis and disease resistance gene Cf-9 in tomato is largely unresolved. Although a great deal of work has been carried out on Cf-genes in tomato, it is still not known how these genes with an extracellular LRR domain activate the signalling and confer resistance. Last year, we cloned the RPP27 gene, an RLP that confers downy mildew resistance in Arabidopsis. By using the extensive molecular and genetic resources developed for Arabidopsis, we propose to investigate the genetic requirements of RPP27-mediated resistance and identify proteins required for activating the defence response. This will involve a mutagenesis approach to genetically dissect the signalling pathway and use the already known disease resistance mutants in the analysis. The expression and the localisation of the gene will be characterised by GFP tagging system and the confocal microscopy. Interacting partners will be identified using tagging and MALDI-MS. We believe this provides a unique opportunity to identify the partners for this orphan receptor protein and understand the role of RLP-mediated defence response. Identification of genetic requirements should enable us to compare the RLP- and NB-LRR mediated defence responses. The interaction between the RPP27 and the partner protein would be the immediate objective following identification of the partner protein(s). The partner protein can be tagged and studied in the same way as with RPP27. If the partner protein is a receptor-like kinase (RLK) protein, as was shown with CLV2, the cytoplasmic kinase domain could be used as a bait in a method such as yeast two hybrid system to identify further interactors including NB-LRR genes. By analogy to Cf-proteins, RPP27 may recognise the corresponding Avr protein secreted by the parasite in the intercellular space or in the extrahaustorial matrix.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	Crop Science, Plant Science
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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