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Functional genomic analysis of the regulation of photosynthetic electron transport

Reference	BB/C508877/1
Principal Investigator / Supervisor	Professor Giles Johnson
Co-Investigators / Co-Supervisors	Dr Patrick Gallois
Institution	The University of Manchester
Department	Life Sciences
Funding type	Research
Value (£)	311,287
Status	Completed
Type	Research Grant
Start date	01/04/2005
End date	31/03/2008
Duration	36 months

Abstract

Developments in Arabidopsis genetics, especially arising from the genome sequencing project are making available new resources for studying the functioning of plants. If these resources are to be fully exploited, analysis needs to be taken to physiological level. The objectives of this project are to develop a new facility for the integrated measurement of chloroplast processes in whole leaves and to apply this to the study of knock-out mutants of Arabidopsis, in order to characterise fundamental processes in the regulation of photosynthesis and, specifically in the regulation of cyclic electron transport around photosystem 1. Long an enigma in photosynthesis research, recent advances make study of cyclic ET especially timely. Advances in optical spectroscopy mean that we are now better able to quantify cyclic ET under a wide variety of conditions. Our understanding of the role of cyclic ET has been advanced by the recent description of mutant of Arabidopsis, pgr5, deficient in this pathway, which is impaired in its ability to cope with environmental stress (Munekage et al., 2002, Cell 110, 361-371). Other insights into the pathway of cyclic ET come from new structural findings (Kurisu et al., 2003, Science 302 1009-1014; Stroebel et al., 2003 Nature 426 413-418). We will perform a detailed spectroscopic, functional analysis of pgr5 and of a variety of T-DNA knock-out mutants predicted to be affected in cyclic ET, in order to test specific hypotheses relating to the regulation of photosynthesis in response to environmental stress.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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