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The role of the molecular chaperones/remodelling factors TIP48 and TIP49 in box C/D snoRNP biogenesis.

Reference	BB/C508069/1
Principal Investigator / Supervisor	Dr Nicholas Watkins
Co-Investigators / Co-Supervisors
Institution	Newcastle University
Department	Inst for Cell and Molecular Biosciences
Funding type	Research
Value (£)	262,355
Status	Completed
Type	Research Grant
Start date	01/01/2005
End date	31/12/2007
Duration	36 months

Abstract

The process of ribonucleoprotein (RNP) biogenesis of both highly complex machines such as the ribosome and spliceosome as well as simpler complexes such as the spliceosomal snRNPs (pre-mRNA splicing) and the nucleolar box C/D snoRNPs (ribosome biogenesis) has been shown to involve a number of ATPases and ATP hydrolysis. We have recently shown that box C/D snoRNPs undergo a highly complex series of protein remodelling steps during biogenesis that are essential for the formation of a stable mature complex. We have also shown that TIP48 and TIP49 are two AAA+ ATPases essential for box C/D snoRNP accumulation/biogenesis in HeLa cells. Our work has led us to hypothesise that these two proteins, which are the only two putative remodelling factors shown to be associated with the box C/D snoRNPs during their biogenesis, are essential for the extensive protein rearrangements. We will use a combination of in vitro biochemical experiments and RNAi-based in vivo approach to define the role of TIP48 and TIP49 in box C/D snoRNP biogenesis. In particular, we will focus on defining the target/substrate proteins of these AAA+ proteins as well as characterising the role ATP hydrolysis/binding plays in these extensive protein rearrangements both in vivo and in vitro.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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