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The role of sulfatase in regulating FGF signalling during myogenesis in Xenopus tropicallis
Reference
BB/C505408/1
Principal Investigator / Supervisor
Dr Mary Pownall
Co-Investigators /
Co-Supervisors
Institution
University of York
Department
Biology
Funding type
Research
Value (£)
237,741
Status
Completed
Type
Research Grant
Start date
01/10/2004
End date
30/06/2008
Duration
45 months
Abstract
My lab has shown previously that the signalling molecule embryonic fibroblast growth factor (eFGF), which is the amphibian homologue of FGF-4, is necessary for the initial activation of XmyoD transcription in myogenic cells. FGF signalling requires heparan sulfate proteoglycans (HSPGs), and this project will investigate any role for the gene called Xtsulf1, that encodes a heparan sulfate 6-0 endosulfates, in modulating FGF signalling using explant studies, gene expression analysis, mis-expression and gene inhibition. While inhibiting gene function in very early embryos is difficult or very time consuming in other vertebrates, the experiments proposed are straight forward in an amphibian model. Cloning Xtsof1 from X. tropicalis, a diploid relative of X. laevis, will simplify the antisense based approach to gene inhibition and provide insight into the vivo role that Xtsulf1 plays in regulating gene expression and signal transduction pathways during early development. The main objectives of this project in (1) to demonstrate a biological activity for Xtsulf1 in modulating FGF signalling and (2) to define an in vivo role for Xtsulf1 in modifying signal transduction and gene expression in the developing embryo.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
Stem Cells, The 3 Rs (Replacement, Reduction and Refinement of animals in research)
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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