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Annexin function in Arabidopsis root hair growth

Reference	BB/C505232/1
Principal Investigator / Supervisor	Professor Julia Davies
Co-Investigators / Co-Supervisors	Professor Nicholas Battey, Professor Colin Brownlee, Dr Neil Macpherson, Professor Alex Webb
Institution	University of Cambridge
Department	Plant Sciences
Funding type	Research
Value (£)	217,962
Status	Completed
Type	Research Grant
Start date	08/11/2004
End date	07/11/2007
Duration	36 months

Abstract

The Arabidopsis root hair contains a single annexin (AtANN1) and so is an ideal single cell system for studying the role of annexins in plant growth, sensing and signalling. We hypothesise that AtANN1 regulates hair growth by functioning as both a plasma membrane calcium channel and a cytosolic peroxidase. To test this, AtANN1 intracellular location as a function of growth will be tracked using a GFP fusion protein. This will tell us whether AtANN1 inserts into the plasma membrane where channel function is anticipated. We shall produce recombinant AtANN1 to test for channel activity in planar lipid bilayers and in vitro peroxidase activity. To link protein activity to cell function we shall investigate the effect of AtANN1 knockout on hair growth, intracellular calcium dynamics and reactive oxygen species production using fluorescent microscopy and electrophysiology.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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