Award details

A systems biology strategy for understanding the genome-wide control of growth rate and metabolic flux in yeast.

Reference	BB/C505140/2
Principal Investigator / Supervisor	Professor Stephen Oliver
Co-Investigators / Co-Supervisors
Institution	University of Cambridge
Department	Biochemistry
Funding type	Research
Value (£)	494,910
Status	Completed
Type	Research Grant
Start date	01/09/2007
End date	30/06/2010
Duration	34 months

Abstract

We shall develop both top-down and bottom-up genome-wide models for the control of the maximum specific grown rate in bakers yeast. Genes with high flux-control coefficients will be identified via haploinsufficiency measurements in turbidostats. Genome-wide metabolite binding and flux/transformation/enzyme kinetic measurements will be carried out mass spectrometrically using protein microarrays obtained from M. Snyder. Intra- and extra-cellular metabolome transcriptome and proteome measurements in selected genetically defined strains will be used, iteratively, to validate the model. Flux-balance modelling will also be used to define specific modulations likely to be most discriminatory between competing models. The result will be the first example in which the controls on growth rate and metabolic fluxes are established on a genome-wide scale.

Summary

unavailable

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	Microbiology, Systems Biology
Research Priority	X – Research Priority information not available
Research Initiative	Proteomics and Cell Function (PCF) [2003-2004]
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

BB/C003608/1 A systems biology strategy for understanding the genome-wide control of growth rate and metabolic flux in yeast

BB/C505140/1 A systems biology strategy for understanding the genome-wide control of growth rate and metabolic flux in yeast.

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