Award details

Gene expression in the Drosophila testis

Reference	BB/C503903/1
Principal Investigator / Supervisor	Professor Helen White-Cooper
Co-Investigators / Co-Supervisors	Dr David Shotton
Institution	University of Oxford
Department	Zoology
Funding type	Research
Value (£)	323,262
Status	Completed
Type	Research Grant
Start date	01/12/2004
End date	31/05/2008
Duration	42 months

Abstract

Comprehensive analysis of gene expression using microarray technology generates large data sets that are becoming fundamental to our understanding of how complex gene networks function in biology. However genome scale analysis of multicellular organisms is confounded by the complexity of tissue- and cell-type specific gene expression. The testis expresses many genes not expressed in any other tissue, and is an excellent, but under exploited, system for studying not only tissue specific gene expression but also fundamental aspects of cell biology ¿ stem cell dynamics, cell cycle, cytoskeletal organisation, membrane remodelling ¿ to name but a few. Se have already conducted a micro-array expression profiling experiment on testis; the aim of this proposal is to accurately describe the spatial and temporal distribution of transcripts of up to 1500 genes in Drosophila testes. We will use RT-PCR then RNA in situ hybridisation to examine the transcript levels and distribution in wild type and certain mutant testes. The use of a set of mutants in the analysis will allow us to investigate the genetic control of the expression of each of the 1500 genes. We will further describe the mutant phenotype in testis of a subset of these genes. All of the data generated will be curated into a custom built Drosophila Testis Gene Expression Database, and will be published on the web in a fully searchable form, available to the entire research community.

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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