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UV-B signalling in Arabidopsis

Reference	BB/C007727/1
Principal Investigator / Supervisor	Professor Gareth Islwyn Jenkins
Co-Investigators / Co-Supervisors
Institution	University of Glasgow
Department	Institute of Biomedical & Life Sciences
Funding type	Research
Value (£)	242,451
Status	Completed
Type	Research Grant
Start date	01/02/2005
End date	31/05/2008
Duration	40 months

Abstract

UV-B induces physiological responses in plants, but very little is known about the mechanisms of UV-B perception and signal transduction. Through isolation and characterisation of an Arabidopsis mutant we have shown that a protein called UVR8 is specifically involved in these processes. UVR8 is structurally very similar to a human protein, RCC1, the guanine nucleotide exchange factor (GEF) for the small GTP-binding protein, Ran. RCC1 binds to chromatin and functions in several processes involving Ran-GTP, including nucleocytoplasmic transport and progression of the cell cycle. However, RCC1 is not known to function in UV-B responses. The aim of this project is to start to understand how UVR8 functions in plant UV-B perception and/or signal transduction. We will (i) establish whether UVR8 has the known properties of RCC1 homologues (nuclear localisation, chromatin binding and GEF activity); (ii) determine which parts of the UVR8 protein are essential to its function by testing complementation of the urv8 mutant with modified UVR8 proteins; (iii) examine whether UVR8 binds to nucleosomes and to specific histones; (iv) establish whether UVR8 functions through association with other proteins in a chromatin complex.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	Plant Science
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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