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Isolation of new potato genes for resistance to Phytophthora infestans from wild diploid Solanum species

Reference	BB/C007522/1
Principal Investigator / Supervisor	Professor Jonathan Jones
Co-Investigators / Co-Supervisors
Institution	University of East Anglia
Department	Sainsbury Laboratory
Funding type	Research
Value (£)	353,382
Status	Completed
Type	Research Grant
Start date	05/09/2005
End date	04/12/2008
Duration	39 months

Abstract

We have identified three new resistance genes to P. infestans (Rpi-oka1, Rpi-oka2 and Rpi-neo1) in wild Solanum species. The main goal of the project is to clone these genes and show that they function when transformed into potato. We will also map with high resolution, and if possible isolate, 5-10 additional Rpi genes. The three high priority Rpi genes have already been mapped to a chromosome arm. The project will take advantage of the forthcoming tomato DNA sequence to accelerate the identification of cosegregating R gene candidates (NB-LRRs or Cf-9- like genes) prior to subjecting them to virus-induced gene silencing (VIGS) of the Rpi gene candidate, and testing silenced plants for loss of resistance. The JIC Genome Centre will make a BAC library from a genetic stock carrying Rpi-oka1 and Rpi-oka2. Rpi-neo1 will be isolated from a binary cosmid library to be made from resistant plants. R gene families shown by VIGs to be required for resistance will be cloned into a transformable binary cosmid vector and used to make transgenic potato var Desiree lines that will be assayed for blight resistance. Genome sequence comparisons between tomato and potato at Rpi gene loci will enrich our understanding of R gene evolution. The ultimate aim of the project is to deploy these multiple Rpi genes in Rpi gene mixtures in the field to test the idea that heterogeneity for R gene composition will promote durable resistance.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	Crop Science, Plant Science
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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