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Dissecting SUF protein-mediated Fe-S cluster biogenesis and repair in Arabidopsis plastids

Reference	BB/C00552X/1
Principal Investigator / Supervisor	Professor Simon Moller
Co-Investigators / Co-Supervisors	Dr Xiang Xu
Institution	University of Leicester
Department	Biology
Funding type	Research
Value (£)	242,967
Status	Completed
Type	Research Grant
Start date	07/08/2005
End date	06/07/2006
Duration	11 months

Abstract

Iron-sulfur (Fe-S) clusters are inorganic cofactors prosthetic groups of Fe-S proteins involved in vital biological processes such as electron transport, redox non-redox catalysis, sensing, signalling and regulation of gene expression. Fe-S cluster biogenesis repair requires an intricate interplay of a number of proteins however in plants little is known regarding this process and possible target Fe-S proteins. In prokaryotes the suf (mobilisation of sulfur) operon (sufABCDSE) is involved in Fe-S cluster biosynthesis repair and we have shown that Arabidopsis plastids contain a complete SUF system, that the plastidic SufC homolog AtMAP7 is an ABC ATPase that can complement SufC-deficiency in E. coli and that AtNAP7 is essential for embryogenesis. The proposed research builds directly on our generated data with the aim of dissecting SUF-mediated Fe-S cluster biogenesis and repair in Arabidopsis. Through inducible RNAi approaches we will define the precise mode of action of AtNAP7 in terms of Fe-S biogenesis and or repair and identify target Fe-S proteins. Furthermore we will characterise the SurfD homolog AtNAP6 at the molecular and biochemical level, define its role during plant development and analyse how AtNAP7 and AtNAP7 act together. The possible involvement of AtNAP6 in auxin responses will also be assessed. Using quantitative proteome analysis we will further identify novel Fe-S proteins that are targets for the SUF-mediated Fe-S cluster biogenesis repair system and explore the possibility that chloroplasts export Fe-S clusters for incorporation into cytosolic apoproteins.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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