Award details

Exploiting the genomic resource for the development of novel carbohydrate binding modules

Reference	BB/C005074/1
Principal Investigator / Supervisor	Dr David Bolam
Co-Investigators / Co-Supervisors	Professor Gideon Davies, Professor Benjamin Davis
Institution	Newcastle University
Department	Inst for Cell and Molecular Biosciences
Funding type	Research
Value (£)	579,783
Status	Completed
Type	Research Grant
Start date	01/10/2005
End date	31/03/2010
Duration	54 months

Abstract

The specific objectives of the project are outlined below: 1. Test the hypothesis that modular enzymes which display unusual catalytic properties contain CBMs that exhibit novel specificities. 2. Determination of the 3-D structures of CBMs identified in Objective 1 that display novel ligand specificities. 3. Use rational design, informed by the 3D structural information obtained in Objective 2, to interrogate the molecular basis for the ligand specificity of the CBMs identified in Objective 1. The project will, in Objective 1, use a) standard pET-based E. coli expression vectors to produce the proteins; b) a combination of macroarray and glycochip technology to screen the binding properties of the CBMs mined from genome database; c) a combination of isothermal titration calorimetry and uv difference spectroscopy to precisely define and quantify binding specificities. In Objective 2 the 3d structure of the proteins will be determined by X-ray crystallography. In Objective 3 site-directed mutagenesis will be carried out using the Quikchange PCR-based based.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	Structural Biology
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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