Award details

Developing applications of allosteric aptamers and bespoke ribo-switches

Reference	BB/C004949/1
Principal Investigator / Supervisor	Professor Peter Stockley
Co-Investigators / Co-Supervisors	Dr Tamara Belyaeva, Professor Ian Hope, Professor Adam Nelson
Institution	University of Leeds
Department	Bionanosciences
Funding type	Research
Value (£)	235,782
Status	Completed
Type	Research Grant
Start date	01/03/2005
End date	29/02/2008
Duration	36 months

Abstract

We are proposing to take advantage of our unique access to a small library of aminoglycoside derivatives (AGDs), that we have already screened for antibiotic activity in E. coli, to create a series of AGD-dependent regulatory tools for the control of gene expression in vivo. This takes advantage of our robotic RNA aptamer facilities and recent discoveries in prokaryotic gene expression, namely ribo-switches. We propose developing these tools by validating them against the met regulon, where we have transcriptome data on knockouts etc. We will then apply them to several refined questions of regulatory control in prokaryotes, as well as investigating their utility in developmental studies in C.elegans.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search