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Developing applications of allosteric aptamers and bespoke ribo-switches
Reference
BB/C004949/1
Principal Investigator / Supervisor
Professor Peter Stockley
Co-Investigators /
Co-Supervisors
Dr Tamara Belyaeva
,
Professor Ian Hope
,
Professor Adam Nelson
Institution
University of Leeds
Department
Bionanosciences
Funding type
Research
Value (£)
235,782
Status
Completed
Type
Research Grant
Start date
01/03/2005
End date
29/02/2008
Duration
36 months
Abstract
We are proposing to take advantage of our unique access to a small library of aminoglycoside derivatives (AGDs), that we have already screened for antibiotic activity in E. coli, to create a series of AGD-dependent regulatory tools for the control of gene expression in vivo. This takes advantage of our robotic RNA aptamer facilities and recent discoveries in prokaryotic gene expression, namely ribo-switches. We propose developing these tools by validating them against the met regulon, where we have transcriptome data on knockouts etc. We will then apply them to several refined questions of regulatory control in prokaryotes, as well as investigating their utility in developmental studies in C.elegans.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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