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Developing applications of allosteric aptamers and bespoke ribo-switches

ReferenceBB/C004949/1
Principal Investigator / Supervisor Professor Peter Stockley
Co-Investigators /
Co-Supervisors
Dr Tamara Belyaeva, Professor Ian Hope, Professor Adam Nelson
Institution University of Leeds
DepartmentBionanosciences
Funding typeResearch
Value (£) 235,782
StatusCompleted
TypeResearch Grant
Start date 01/03/2005
End date 29/02/2008
Duration36 months

Abstract

We are proposing to take advantage of our unique access to a small library of aminoglycoside derivatives (AGDs), that we have already screened for antibiotic activity in E. coli, to create a series of AGD-dependent regulatory tools for the control of gene expression in vivo. This takes advantage of our robotic RNA aptamer facilities and recent discoveries in prokaryotic gene expression, namely ribo-switches. We propose developing these tools by validating them against the met regulon, where we have transcriptome data on knockouts etc. We will then apply them to several refined questions of regulatory control in prokaryotes, as well as investigating their utility in developmental studies in C.elegans.

Summary

unavailable
Committee Closed Committee - Genes & Developmental Biology (GDB)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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