Award details

Division versus differentiation - an integrated biology approach

Reference	BB/C004108/1
Principal Investigator / Supervisor	Professor Anna Philpott
Co-Investigators / Co-Supervisors
Institution	University of Cambridge
Department	Oncology
Funding type	Research
Value (£)	203,122
Status	Completed
Type	Research Grant
Start date	01/03/2005
End date	29/02/2008
Duration	36 months

Abstract

This application addresses the fundamental question of how the balance between cell division and differentiation is achieved during the development of a model tissue and organ. It will establish whether cell cycle regulators control the differentiation process directly over and above their ability to control proliferation. The project focuses on the non-redundant cdk inhibitor p27Xic1 and the cyclinA/Cdk2 complex that is regulated by p27Xic1. It aims to establish their roles in the differentiation and patterning of the epidermis and the pronephric kidney in the model organism Xenopus laevis. The project aims to test the hypothesis that the cdk inhibitor p27Xic1 regulates epidermal and pronephric differentiation by perturbing levels of expression by the over-expression of wild-type or mutant mRNA or the morpholino oligonucleotide depletion of p27Xic1. Further it aims to test the hypothesis that the level of cyclin A/cdk2 is the critical regulator of epidermal and pronephric differentiation, using over-expression and knock-down strategies. Finally it aims to define the pattern of proliferation in the embryonic epidermis and the developing pronephros and the pattern of apoptosis in the developing pronephros throughout key developmental stages. The project will provide a novel integrated biology approach to a fundamental question in cell and developmental biology, which cannot be addressed in higher vertebrates due to the established functional redundancy of the cdki¿s. Joint with BB/C00406X/1

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

BB/C00406X/1 Division versus differentiation - an integrative biology approach

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