Award details

A systems biology strategy for understanding the genome-wide control of growth rate and metabolic flux in yeast

Reference	BB/C003608/1
Principal Investigator / Supervisor	Professor Pedro Mendes
Co-Investigators / Co-Supervisors	Professor Simon James Gaskell
Institution	The University of Manchester
Department	Chemistry
Funding type	Research
Value (£)	720,593
Status	Completed
Type	Research Grant
Start date	18/04/2005
End date	17/11/2009
Duration	55 months

Abstract

Bakers yeast Saccharomyces cerevisiae is a major organism in a number of industries, from brewing and baking to pharmaceuticals, and was the first eukaryotic organism to have its genome sequenced. This makes it a central model organism in modern biology, and it is important including for the development of antifungal agents to know what controls the rate at which it can grow. More generally, we may better claim to understand its biology if we can model its behaviour mathematically. This requires that we know the amounts and kinetic properties of each enzyme. We shall develop methods for estimating these on a genome-wide scale. Competition experiments using strains, each of which has had one copy of a protein-encoding gene removed from its genome, we can determine those which exert the most control over yeasts growth rate. By measuring all the metabolites, RNA molecules and protein levels, we shall be able to develop and test iteratively a mathematical model which can describe all the metabolic fluxes. This will produce, for the first time, an experimentally validated model of the gross dynamics and control of flux in a eukaryotic organism.

Summary

unavailable

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	Microbiology, Systems Biology
Research Priority	X – Research Priority information not available
Research Initiative	Proteomics and Cell Function (PCF) [2003-2004]
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

BB/C505140/1 A systems biology strategy for understanding the genome-wide control of growth rate and metabolic flux in yeast.

BB/C505140/2 A systems biology strategy for understanding the genome-wide control of growth rate and metabolic flux in yeast.

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