Award details

Translational regulation of L- and N-myc by internal ribosome entry; trans-acting factor requirements structure and mechanisms

Reference	BB/C000390/1
Principal Investigator / Supervisor	Professor Anne Willis
Co-Investigators / Co-Supervisors
Institution	University of Nottingham
Department	Sch of Pharmacy
Funding type	Research
Value (£)	388,178
Status	Completed
Type	Research Grant
Start date	01/02/2005
End date	31/01/2008
Duration	36 months

Abstract

Protein synthesis can be initiated by two distinct mechanisms namely cap-dependent scanning and internal ribosome entry. It is becoming apparent that the latter mechanism is important in the control of synthesis of cellular mRNAs whose protein products are involved in the control of cell growth, programmed cell death and in development. Recent data suggests that as many as 4000 mRNAs have the potential to be translated by this mechanism. For internal ribosome entry to occur a complex structural element termed an internal ribosome entry segment (IRES) must be present in the 5 prime UTR of the mRNA. In this grant I propose to identify the non-canonical trans-acting proteins which interact with the N and L-myc IRESs to determine the effect that these proteins elicit on the structure of the IRESs, and do determine how the L-myc and N-myc IRESs interact with the ribosome. This application is based upon work carried out in my laboratory over the last three years where we have derived structures for the L-myc and N-myc IRESs and have carried out mechanistic studies. The experiments proposed herein complement other studies in my laboratory and the overall goal of my work is towards understanding how cellular IRESs function and to determine whether there are common and distinct features of structure/function/proteins that are shared by IRESs whose protein products have related activities.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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