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The structural basis of gene regulation in a novel restriction-modification system
Reference
B20404
Principal Investigator / Supervisor
Professor Geoff Kneale
Co-Investigators /
Co-Supervisors
Professor Jon Cooper
Institution
University of Portsmouth
Department
Sch of Biological Sciences
Funding type
Research
Value (£)
208,453
Status
Completed
Type
Research Grant
Start date
01/11/2003
End date
31/10/2006
Duration
36 months
Abstract
To avoid cell death, it is vital that the expression of restriction-modification genes is subject to strict temporal control such that restriction activity is delayed with respect to methylation. This delay is accomplished by means of a regulator protein, the C-protein. We have recently purified the C-protein from the AhdI R-M system and obtained crystals that diffract to 2.2 angstrom resolution. We propose to solve the structure of the protein and relevant protein-DNA complexes by X-ray crystallography, to define the precise DNA binding sequence(s) recognised by the protein, and to establish and subsequently modify the protein-protein and protein-DNA interactions that underpin this intricate genetic switch.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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