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Mechanism of a flap endonuclease - one or two metal ions?

Reference	B20079
Principal Investigator / Supervisor	Professor Jane Grasby
Co-Investigators / Co-Supervisors	Professor Nicholas Williams
Institution	University of Sheffield
Department	Chemistry
Funding type	Research
Value (£)	192,740
Status	Completed
Type	Research Grant
Start date	26/07/2004
End date	25/07/2007
Duration	36 months

Abstract

Flap endonucleases are metallonucleases that play critical roles in DNA replication and repair. It is likely that they represent a new mechanistic class of metalloenzymes. To probe the role that metal ions play in this reaction, we will undertake experiments designed to investigate their potential involvement in either catalysis or maintaining structure. The effects of varying the cofactor, modification of the electronic properties of the leaving group, and the number of metals required for reaction will all be investigated. In addition, model studies will test the underlying assumptions on which mechanistic proposals are built. Together these studies will allow us to make mechanistic proposals for this important class of enzymes.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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