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Molecular investigation of nisin/lipid II complexes: a novel mechanism of membrane recognition for antibiotic design

ReferenceB20039
Principal Investigator / Supervisor Dr Boyan Bonev
Co-Investigators /
Co-Supervisors
Institution University of Nottingham
DepartmentSch of Biomedical Sciences
Funding typeResearch
Value (£) 186,046
StatusCompleted
TypeResearch Grant
Start date 01/02/2004
End date 31/01/2007
Duration36 months

Abstract

Resistance to antibiotics is a major problem in the control of pathogenic bacteria. Penicillin tolerance is common and vacomycin resistance has been reported. An alternative exists in the antibiotic nisin, a potent bactericidal peptide. During bactericidal membrane recognition nisin targets peptidoglycan intermediate Lipid II located in the outer leaflet, where the two form lytic oligomeric complexes. We have observed such complexes using solid state NMR and propose a detailed structural study of the nisin/Lipid II complexes using solid state NMR and neutron reflectivity. The structure of Lipid II in the complex will be used as a new template for the design of highly selective antibiotics. Nisin is proposed as the archetype of this new class.

Summary

unavailable
Committee Closed Committee - Biomolecular Sciences (BMS)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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