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Structure and mechanism of a DNAzyme RNaseA mimic
Reference
B20026
Principal Investigator / Supervisor
Dr David Mark Williams
Co-Investigators /
Co-Supervisors
Professor Jane Grasby
Institution
University of Sheffield
Department
Chemistry
Funding type
Research
Value (£)
197,240
Status
Completed
Type
Research Grant
Start date
08/10/2003
End date
07/10/2006
Duration
36 months
Abstract
A trans-cleaving DNAzyme equipped with primary amino and imidazole groups capable of metal-ion independent sequence-specific cleavage of RNA and designed to function as an RNase mimic will be developed. This will build on studies involving a corresponding cis-cleaving DNAzyme. An investigation of the mechanism and substrate specificity of the trans-cleaving ribozyme will be undertaken and will involve the study of pH-rate profiles of the wild-type and mutant DNAzymes. This will be combined with enzymatic and chemical probing of the secondary structure of the DNAzyme complexed to a non-cleavable substrate.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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