Award details

Conformations and binding preferences of the DNA Holliday junction

Reference	B19997
Principal Investigator / Supervisor	Professor Christine Janet Cardin
Co-Investigators / Co-Supervisors
Institution	University of Reading
Department	Chemistry
Funding type	Research
Value (£)	170,423
Status	Completed
Type	Research Grant
Start date	01/01/2004
End date	31/12/2006
Duration	36 months

Abstract

This proposal seeks support for X-ray crystallographic and complementary studies of the Holliday junction structure in DNA oligomers. Our current work shows that several X-stacked structures can be obtained with several cation and sequence specific binding sites, with possible conformation dependence on both seqeunce and cation binding. The junction is known to have high affinity for intercalating drugs and proteins such as p53. We propose to use a combination of competitive dialysis, mass spectrometry and X-ray diffraction to provide accurate structural information on the effect of intercalation (particularly bis-intercalation) on Holliday junction geometry. Native or intercalated p53-binding sequences may spontaneously form junctions.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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