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Biophysical studies on the dissociation and assembly of DNA G-quadruplexes

Reference	B19978
Principal Investigator / Supervisor	Dr Ihtshamul Haq
Co-Investigators / Co-Supervisors
Institution	University of Sheffield
Department	Chemistry
Funding type	Research
Value (£)	251,412
Status	Completed
Type	Research Grant
Start date	17/11/2003
End date	16/11/2006
Duration	36 months

Abstract

Four-stranded DNA structures (G-quadruplexes) are important and worth studying because they have been implicated in a number of biologically significant events in vivo. G-quadruplexes are structurally well characterised, however the energetic/kinetic factors that drive quadruplex formation are less well understood. In this proposal the aim is to investigate the thermodynamics of quadruplex folding/unfolding using direct calorimetric methods supplemented with secondary structure probes (CD). The influence of metal ions on quadruplex stability, especially in terms of biologically relevant mixed Na+/K+ environments will be examined. We will also investigate the role of water and hydration effects on quadruplex stability as well as the effect of loop mutations. These data will enable sequence and cation dependant conformational effects to be better predicted.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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