Award details

Structural and functional analysis of ammonium transport proteins

Reference	B19934
Principal Investigator / Supervisor	Professor Per Bullough
Co-Investigators / Co-Supervisors	Professor Mike Merrick
Institution	University of Sheffield
Department	Molecular Biology and Biotechnology
Funding type	Research
Value (£)	219,262
Status	Completed
Type	Research Grant
Start date	01/10/2003
End date	30/09/2006
Duration	36 months

Abstract

The Amt family of high-affinity ammonium transport proteins is conserved in bacteria, plants and animals, and includes the human Rhesus proteins. We have developed the Escherichia coli AmtB protein as a model for this family. It can be purified to homogeneity and preliminary experiments have given 2D crystals that diffract to 9 angstrom. The objectives of the project are: (1) to produce high-resolution 2D and 3D structures of Amt proteins, and of an AmtB-GlnK complex by EM or X-ray crystallography; (2) to use mutagenesis of AmtB to identify specific residues involved in ammonium binding and transport and thereby to elucidate the mode of action of the protein; (3) to investigate the mode of action of AmtB reconstituted into liposomes. (Joint with grant number 19932).

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

B19932 Structural and functional analysis of ammonium transport proteins

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