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Structural analysis of the REF mRNA export factor using NMR

Reference	B19806
Principal Investigator / Supervisor	Professor Stuart Wilson
Co-Investigators / Co-Supervisors	Dr Alexander Golovanov, Professor Lu-Yun Lian
Institution	University of Sheffield
Department	Molecular Biology and Biotechnology
Funding type	Research
Value (£)	161,660
Status	Completed
Type	Research Grant
Start date	07/06/2004
End date	06/06/2006
Duration	24 months

Abstract

REF is a core component of the TREX and exon junction protein complexes. It associates with a number of different proteins in these complexes and RNA is essential for mRNA export in yeast. We have identified conditions suitable for determination of the structure of REF by NMR techniques. We will determine the complete structure of the REF + and - RNA using NMR. We will also define the residues involved in the interactions with proteins such as Tap, Y14/MAGOH, DDX39 and ORF57 using chemical shift mapping. These studies should lead to structural insights into how this protein can interact with so many partners and RNA through its binding domains and will provide the first structure of a complete mRNA export factor which binds RNA.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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