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Investigation of the architecture of ionotropic receptors using atomic force microscopy
Reference
B19797
Principal Investigator / Supervisor
Professor John Edwardson
Co-Investigators /
Co-Supervisors
Dr Robert Henderson
Institution
University of Cambridge
Department
Pharmacology
Funding type
Research
Value (£)
158,480
Status
Completed
Type
Research Grant
Start date
03/03/2004
End date
02/03/2007
Duration
36 months
Abstract
Fast synaptic transmission involves the operation of ionotropic receptors, which are usually composed of at least two types of subunit. Although the subunit stoichiometry is sometimes known, the arrangement of subunits around the receptor rosette is usually undetermined. We plan to investigate the subunit architecture of three ionotropic receptors ¿ the GABA A receptor, the 5HT3 receptor and the P2X receptor for ATP ¿ all of which have considerable physiological and therapeutic significance. We will produce receptors containing epitope-tagged subunits and incubate these with anti-epitope antibodies. Imaging of the receptor-antibody complexes using atomic force microscopy will enable us to deduce the structures of the receptors.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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