Award details

The putative yeast linker histone Hho1p: structure and interaction with DNA and chromatin

Reference	B19489
Principal Investigator / Supervisor	Professor Dame Jean Olwen Thomas
Co-Investigators / Co-Supervisors	Dr Katherine Stott
Institution	University of Cambridge
Department	Biochemistry
Funding type	Research
Value (£)	290,636
Status	Completed
Type	Research Grant
Start date	01/10/2003
End date	31/05/2007
Duration	44 months

Abstract

The overall aim is to understand the role of nuclear protein Hho1p in Saccharomyces cerevisiae, and in particular to evaluate it as a candidate linker histone. Specific aims are (1) To determine how Hho1p binds to chromatin, and whether it plays an nucleosome bridging role, utilizing its two globular domains. (2) To determine the nuclear stoichiometry of Hho1p and whether it is clustered in chromosomes, and to investigate its presence on promoters and coding sequences of various genes. (3) To determine the structure of the two globular domains, GI and GII, by NMR (already initiated) and to use NMR to investigate reasons for the different stabilities of the two domains, and to identify residues involved in DNA binding. The feasibility of NMR structure determination of complexes of GI and or GII with four- way DNA junctions will also be assessed.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search