Award details

The biosynthesis of coenzyme F430 in methanothermobactor thermautotrophicus

Reference	B19356
Principal Investigator / Supervisor	Professor Peter Heathcote
Co-Investigators / Co-Supervisors	Dr Evelyne Deery, Professor Richard Pickersgill, Dr Stephen Rigby
Institution	Queen Mary University of London
Department	Sch of Biological and Chemical Sciences
Funding type	Research
Value (£)	263,600
Status	Completed
Type	Research Grant
Start date	01/01/2004
End date	31/12/2005
Duration	24 months

Abstract

We will elucidate the biosynthesis of coenzyme F430, the yellow tetrapyrrole-derived cofactor required in the terminal step of methanogenesis and which plays a pivotal role in the production of a trillion tons of methane gas per year. Using a cell free system we will determine the intermediates formed between precorrin-2 and coenzyme F430 and attempt to isolate the enzymes. Using a substractive genomic bioinformatics approach we have generated a shortlist of 29 proteins, which is likely to contain all the F430 biosynthetic enzymes. These 29 proteins will be overproduced recombinantly, integrated into a structural genomics programme of research, and also used to investigate their role in the biosynthesis of coenzyme F430.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

B19356/2 The biosynthesis of coenzyme F430 in methanothermobactor thermautotrophicus

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