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Analysing a mechano-chemical motor controlling transcriptional initiation

Reference	B19279
Principal Investigator / Supervisor	Professor Peter Stockley
Co-Investigators / Co-Supervisors	Professor Martin Buck, Professor David Smith
Institution	University of Leeds
Department	Inst of Molecular & Cellular Biology
Funding type	Research
Value (£)	227,944
Status	Completed
Type	Research Grant
Start date	01/09/2003
End date	31/08/2006
Duration	36 months

Abstract

The goals of this project are to use both ensemble and single molecule fluorescence measurements to - 1) examine the sigma54-promoter DNA recognition event and determine the molecular mechanism by which these contacts change during the isomerisation reaction; 2) repeat the measurements from (1) but in the context of the RNA polymerase holoenzyme; 3) characterise the sigma54-Pspf activator recognition event and determine the molecular mechanism by which these contacts change during NTP-hydrolysis mediated isomerisation; 4) to develop a single molecule system in which entire activation process can be observed in an appropriately complex content, i.e. in the presence of the activation loop of DNA and the RNAP holoenzyme, and 5) integrate these mechanistic studies with the structural information coming from the IC group to provide the most detailed description to date of a transcriptional activation event.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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