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Mechanistic studies of a light-driven enzyme; catalysis of hydride and proton transfers by protochlorophyllide reductase

Reference	B19270
Principal Investigator / Supervisor	Krebs Professor of B Christopher Hunter
Co-Investigators / Co-Supervisors	Professor Peter Heathcote, Dr Derren Heyes, Dr Stephen Rigby
Institution	University of Sheffield
Department	Molecular Biology and Biotechnology
Funding type	Research
Value (£)	228,648
Status	Completed
Type	Research Grant
Start date	09/04/2003
End date	09/04/2006
Duration	36 months

Abstract

The catalytic cycle of the chlorophyll biosynthetic enzyme protochlorophyllide reductase (POR) is driven by light. It provides a unique opportunity to trap intermediates along a reaction pathway following initiation of catalysis by illumination at low temperatures, and to measure enzyme kinetics on a subpicosecond timescale. The aims of this proposal are to dissect the catalytic mechanism further by elucidating the molecular nature of enzyme-bound intermediates and to determine the stages in the reaction that proton and hydride transfers occur. This will be achieved by using a combination on EPR/ENDOR spectroscopy, site-directed mutagenesis, the use of Pchlide analogues, and FTIR, Raman, stopped-flow and ultrafast spectroscopy.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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