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The enzymology of thiamine biosynthesis
Reference
B19231
Principal Investigator / Supervisor
Professor Peter Roach
Co-Investigators /
Co-Supervisors
Institution
University of Southampton
Department
Sch of Chemistry
Funding type
Research
Value (£)
213,664
Status
Completed
Type
Research Grant
Start date
01/10/2003
End date
30/09/2006
Duration
36 months
Abstract
This project aims to understand how E. coli assembles the cyclic thiazole portion of thiamine from acyclic precursors, 1- deoxyxylulose, cysteine and tyrosine. A novel radical dependent mechanism for the cyclisation leading to the thiazole is proposed. To test this hypothesis, the key biosynthetic enzymes required for thiazole formation and the reactions that they catalyse will be investigated. An in vitro assay for thiamine biosynthesis will be used to identify the essential substrates, cofactors and proteins. A combination of biochemical and spectroscopic approaches will be used to determine the mechanism of the sulphur insertion and the cyclisation steps leading to the thiazole.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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