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Enzymatic organo-fluorine synthesis. Isolation of the enzymes of fluorometabolite biosynthesis in streptomyces cattleya

Reference	B19112
Principal Investigator / Supervisor	Professor David O'Hagan
Co-Investigators / Co-Supervisors	Dr Jonathan Spencer
Institution	University of St Andrews
Department	Biology
Funding type	Research
Value (£)	208,868
Status	Completed
Type	Research Grant
Start date	01/10/2003
End date	30/09/2006
Duration	36 months

Abstract

Support is requested to explore the enzymology and molecular biology of fluorometabolite biosynthesis in S. cattleya. Key objectives are to clone and over-express the recently discovered fluorination enzyme. The programme will extend to the identification of the remaining unknown enzymes involved in fluorometabolite biosynthesis. Purification of these enzymes for sequencing, cloning and over-expression will be pursued. A stereochemical analysis of the conversion of ATP and fluoride ion to 5 -FDA (via SAM) will be studied with the purified fluorinase to explore the mechanism of biological fluorination. Sequencing of the complete fluorometabolite biosynthesis gene cluster is a clear objective of the programme. (Joint with grant 19113).

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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