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Defining substrate binding specificity in ascorbate peroxidase
Reference
B19083
Principal Investigator / Supervisor
Professor Emma Raven
Co-Investigators /
Co-Supervisors
Institution
University of Leicester
Department
Chemistry
Funding type
Research
Value (£)
207,908
Status
Completed
Type
Research Grant
Start date
19/05/2003
End date
19/05/2006
Duration
36 months
Abstract
We seek a complete description of the substrate binding specificity in ascorbate peroxidase (APX). APX is an excellent model for probing these questions since its substrate binding properties are diverse and place it at the interface between the class I and class III haem peroxidases. The specific aims are: (i) to probe binding of ascorbate at the gamma-meso haem position; (ii) to identify the location of the second ascorbate binding site in APX; (iii) to examine the role of hydrophobic interactions in determining aromatic substrate binding affinity and (iv) to engineer a cytochrome c binding site into APX. The information will underpin our general understanding of the different functional activities exhibited by the haem peroxidases.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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