Award details

P450RhF - the first example of a new class of P450 from Rhodococcus sp.

Reference	B18767
Principal Investigator / Supervisor	Professor Stephen Chapman
Co-Investigators / Co-Supervisors	Professor Sabine Flitsch, Dr Gareth Roberts, Professor Nicholas Turner
Institution	University of Edinburgh
Department	Sch of Chemistry
Funding type	Research
Value (£)	243,264
Status	Completed
Type	Research Grant
Start date	01/04/2003
End date	01/04/2006
Duration	36 months

Abstract

We have recently reported the sequence of a gene from Rhodococcus sp. encoding a P450RhF which is of unique primary structural organisation: analysis of the translation product revealed that the P450 is fused to a reductase domain at the C-terminus which displays sequence conservation to dioxygenase reductase proteins. Ethoxycoumarin was found to be a substrate for P450RhF, when over-expressed in E. coli. Fused P450 monooxygenases are of great interest for mechanistic studies, in particular studies of electron transport. In addition, they have great potential in the application of biohydroxylations to organic synthesis. The aim of the present proposal is to purify and characterise this novel enzyme.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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