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Structure and function of a novel dioxygenase
Reference
B18665
Principal Investigator / Supervisor
Professor Jon Cooper
Co-Investigators /
Co-Supervisors
Dr David Hopper
,
Professor Stephen Wood
Institution
University of Southampton
Department
Centre for Biological Sciences
Funding type
Research
Value (£)
56,204
Status
Completed
Type
Research Grant
Start date
01/04/2003
End date
31/03/2004
Duration
12 months
Abstract
This proposal is to analyse a bacterial dioxygenase namely 2,4- dihydroxyacetophenone dioxygenase (or DAD) which is involved in the decomposition of aromatic compounds from plants. This dioxygenase is of interest since it catalyses the cleavage of an aliphatic side-chain rather than the more usual aromatic ring fission and it sequence shows no similarity to any other protein. Our objectives are to determine its three-dimensional structure by X-ray crystallography and to analyse complexes of the enzyme and site- directed mutants for an improved understanding of its catalytic mechanism. There are implications for the removal of aromatic pollutants in the environment and for the production of chemicals by biotransformations.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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