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Characterisation of haem-protein cross-linked respiratory proteins: investigation of mechanisms for enhanced pro-oxidant activity

Reference	B18662
Principal Investigator / Supervisor	Professor Mike Wilson
Co-Investigators / Co-Supervisors	Professor Christopher Cooper, Professor Peter Nicholls
Institution	University of Essex
Department	Biological Sciences
Funding type	Research
Value (£)	223,696
Status	Completed
Type	Research Grant
Start date	01/04/2003
End date	01/04/2006
Duration	36 months

Abstract

The formation of a covalent bond between the haem and the globin of myoglobin and haemoglobin is an explicit marker for respiratory haem protein-induced oxidative stress in vivo. These modified forms of haem proteins have been implicated in the pathology for several diseases because of their ability to catalyse the oxidation of both free and membrane associated lipids. Building on our previous work we intend to: i) investigate the molecular mechanisms of these oxidative reactions; ii) examine the function of haem to protein cross-linked myoglobin and haemoglobin formation and the mechanisms of their formation; iii) elucidate the reasons for the enhanced pro-oxidant activity of cross-linked Mb contrasting this with Haemoglobin that shows no such enhancement. This work will involve a wide range of biophysical methods coupled with the use of suitable site directed mutants.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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