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Spectroscopic and kinetic studies to elucidate the structure and mechanism of the plant alternative oxidase
Reference
B18603
Principal Investigator / Supervisor
Professor Anthony Moore
Co-Investigators /
Co-Supervisors
Institution
University of Sussex
Department
Sch of Life Sciences
Funding type
Research
Value (£)
101,552
Status
Completed
Type
Research Grant
Start date
01/04/2003
End date
31/03/2005
Duration
24 months
Abstract
The plant alternative oxidase (AOX) is a mitochondrial respiratory enzyme that is poorly characterised in terms of its structure and function. We will study AOX mechanism both at steady state and during the transient reaction phase, using a protein purified from Arum maculatum. Stopped-flow optical spectroscopy will be performed to reveal key catalytic intermediates, which will be trapped kinetically for further characterisation by EPR spectroscopy. FTIR difference spectroscopy will be used to probe the molecular structure of both the active and quinone-binding site(s) of the AOX. In addition, AOX will be over-expressed in Escherichia coli to allow structural and mechanistic comparison between the wild-type enzyme and site-directed mutants.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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