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Probing conformational dynamics in signalling EGF receptors via multidimensional single-molecule fluorescence microscopy
Reference
B18301
Principal Investigator / Supervisor
Professor Marisa Martin-Fernandez
Co-Investigators /
Co-Supervisors
Dr Barry Barraclough
,
Mr Gareth Derbyshire
,
Dr John Smith
,
Dr M Tobin
Institution
STFC - Laboratories
Department
STFC - Laboratories Department
Funding type
Research
Value (£)
150,052
Status
Completed
Type
Research Grant
Start date
15/02/2003
End date
15/02/2005
Duration
24 months
Abstract
We propose to study the conformational changes in the epidermal growth factor receptor (EGFR) during signal transduction that initiates signalling cascades leading to cell growth and division. Receptor conformational changes are masked in observations of ensemble- averaged populations. Hence, we propose to undertake this research by collecting time-resolved single-molecule fluorescence spectroscopy and imaging data on a physiologically relevant group of receptors in the cell. Single-molecule observations not only provide structural information on changes in the distance and orientation of receptor domains, but also allow the determination of the degree of structural heterogeneity within an ensemble to reveal individual stochastic events.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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