Award details

Oligonucleotides containing phosphorothiolate linkages: synthesis and applications

Reference	B18146
Principal Investigator / Supervisor	Professor Richard Cosstick
Co-Investigators / Co-Supervisors	Dr Julie Fisher
Institution	University of Liverpool
Department	Chemistry
Funding type	Research
Value (£)	176,252
Status	Completed
Type	Research Grant
Start date	01/05/2003
End date	31/07/2006
Duration	39 months

Abstract

We have previously shown that a single 3-S-phosphorothiolate linkage incorporated into an oligonucleotide stabilises duplexes with RNA and that these analogues are important probes in studying DNA cleavage processes; however their synthesis remains poorly developed. We plan to develop efficient and automated procedures for the synthesis of ODNs that contain multiple phosphorothiolate linkages in any sequence context. The properties of these modified ODNs (thermal duplex stability with a complementary RNA strand and their ability to stimulate RNase H, will be related to structural changes. These analogues will also be applied to mechanistic studies on E.coli VSR endonuclease.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search