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Engineering of chain transfer in polyketide and peptide-polyketide synthase multienzymes
Reference
B18119
Principal Investigator / Supervisor
Professor Peter Leadlay
Co-Investigators /
Co-Supervisors
Dr Jonathan Spencer
,
Professor Kira Weissman
Institution
University of Cambridge
Department
Biochemistry
Funding type
Research
Value (£)
743,500
Status
Completed
Type
Research Grant
Start date
01/01/2003
End date
31/12/2006
Duration
48 months
Abstract
This project aims to define the structural determinants of intermodular recognition in antibiotic-producing modular polyketide synthases (PKSs) and in mixed peptide-polyketide synthases (PKS-NRPSs). We aim to determine the solution structure of both partner domains responsible for inter-multienzyme docking in a typical modular PKS; we aim to study whether docking specificity can be rationally altered, by using docking domain swaps or by substitutions of individual amino acid residues; we aim to construct chimaeric multienzymes in which an aminoacyl chain may be transferred from an NRPS module to PKS modules; finally, we aim to produce novel amino acid- containing polyketides both in vitro and in vivo.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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