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Binding of substrates drugs and antibiotics to membrane transport proteins elucidated by solid state NMR

Reference	B17940
Principal Investigator / Supervisor	Professor Peter Henderson
Co-Investigators / Co-Supervisors	Professor Stephen Baldwin, Dr Adrian Brough, Professor Maurice Gallagher, Dr R Herbert, Professor Simon Patching, Professor Anthony Watts
Institution	University of Leeds
Department	Inst of Molecular & Cellular Biology
Funding type	Research
Value (£)	187,672
Status	Completed
Type	Research Grant
Start date	12/05/2003
End date	11/07/2006
Duration	38 months

Abstract

We will exploit recent advances in solid state NMR technology, synthetic chemistry and overexpression and mutagenesis of bacterial nucleoside and drug (NupC, NupG) and multidrug or antibiotic (NorA) membrane transport proteins for the following objectives: (1) determine the conformation(s) and mobilities of ligands bound to the proteins; (2) define the ligand-protein interactions and the architectures of the binding sites; (3) define the partition of lipophilic substrates between solution, membrane and protein; (4) illuminate conformational changes in the proteins occurring upon ligand binding. The application of established and leading-edge solid state NMR techniques to understanding ligand binding in particular, and membrane proteins in general, will be advanced.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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