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High-throughput crystallographic studies of membrane transporters

Reference	B17935
Principal Investigator / Supervisor	Professor Peter Henderson
Co-Investigators / Co-Supervisors	Professor Bernadette Byrne, Professor So Iwata, Professor Simon Phillips
Institution	University of Leeds
Department	Inst of Molecular & Cellular Biology
Funding type	Research
Value (£)	495,772
Status	Completed
Type	Research Grant
Start date	01/10/2002
End date	30/06/2006
Duration	45 months

Abstract

We aim to determine, by X-ray crystallography, the three- dimensional structure at atomic resolution of at least one membrane transport protein of the Multi Facilitator Superfamily (MFS). We will apply a high throughput crystallisation approach using various MFS proteins. We have already overexpressed and purified 10 MFS transporters, and instituted a protein factory to produce each protein in 2-25 mg batches regularly. To achieve the throughput required, initial crystallisation screening will be automated. In preliminary trials, we have already obtained crystals from 4 different MFS proteins including the one diffracting up to 6 Angstrom.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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