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Structural and functional studies of bluetongue virus non-structural protein 2 (NS2(ViP)) and outer capsid proteins VP2 and VP5

ReferenceB17761
Principal Investigator / Supervisor Professor David Stuart
Co-Investigators /
Co-Supervisors
Dr Andrew Metaxas
Institution University of Oxford
DepartmentWellcome Trust Centre for Human Genetics
Funding typeResearch
Value (£) 80,828
StatusCompleted
TypeResearch Grant
Start date 02/07/2002
End date 01/07/2005
Duration36 months

Abstract

The bluetongue virus inclusion body matrix protein, NS2(ViP), is important in virus genome assembly and morphogenesis. Small crystals (0.05 mm long) of bacterial expressed NS2(ViP) generated weak X-ray diffraction data to beyond 4 angstrom resolution. Crystallisation conditions and diffraction data will be improved to determine the protein's atomic structure. Difference imaging will identify NS2(ViP) binding sites for RNAs, nucleotides, metal ions etc. The role of NS2(ViP) in control of translation, packaging, helicase activity, sequestration of viral RNAs etc. will also be studied. Purified BTV infectious subviral particles (ISVP), in which outer capsid protein VP2 is cleaved, have produced fragile crystals, but as yet no useable diffraction data. The structure of the virus and ISVP outer capsid proteins will be studied by crystallography and cryo-electron microscopy. (Joint with grant 17386).

Summary

unavailable
Committee Closed Committee - Biomolecular Sciences (BMS)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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