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Using C. reinhardtii as a model system for determining the macromolecular structure of PSI and PSII by high resolution electron microscopy
Reference
B17532
Principal Investigator / Supervisor
Dr Jon Nield
Co-Investigators /
Co-Supervisors
Professor James Barber
Institution
Imperial College London
Department
Biological Sciences
Funding type
Research
Value (£)
203,160
Status
Completed
Type
Research Grant
Start date
01/01/2003
End date
01/01/2006
Duration
36 months
Abstract
The transformable green alga, C. reinhardtii, will be used as a model system to obtain structural information about the macromolecular organisation of photosystem I (PSI) and photosystem II (PSII), in higher plants and other photosynthetic eukaryotic organisms that contain chlorophyll a:b light harvesting complexes (LHC). Using electron cryomicroscopy and single particle analyses, a 3D structure of the LHCII-PSII supercomplex will be determined to the highest possible resolution. Related studies will be conducted to isolate and characterise an LHC1-PSI supercomplex, initially by negative stain electron microscopy (EM) and then by cryo-EM. Complementary analyses will also be carried out using mutants of Chlamydomonas lacking PSI, PSII and LHCI and II subunits in order to aid the interpretation of the 3D maps obtained.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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